Shilpa Balar, Esha Joshi, Urja Desai
Currently HER-2/neu
status in breast cancer is categorized by immunohistochemistry (IHC) and fluorescent in situ hybridization (FISH) in clinical practice. The
amplification of the HER-2/neu gene has a prognostic significance.
Quantitative determination of HER-2/neu gene
amplification by qPCR or
over-expression by qRT-PCR is not routinely used, whereas qPCR based assays can be an alternative method to evaluate
HER-2/neu status. We searched
literature and databases including
the National Institutes of Health’s (NIH), National Library of Medicine (NLM),
The National Center for Biotechnology
Information (NCBI), PubMed, and Google Scholar. The current study aimed to identify
the correlation between FISH, IHC, and real-time-PCR reaction in measuring
HER-2/neu expression in breast
cancer.
Breast cancer, FISH, HER2/neu, IHC, Quantitative PCR, Quantitative RT-PCR.
VOL.14, ISSUE No.1, March 2022