Towards Excellence

(ISSN No. 0974-035X)
(An indexed refereed & peer-reviewed journal of higher education)
UGC-MALAVIYA MISSION TEACHER TRAINING CENTRE GUJARAT UNIVERSITY

MULTIMODALITY TESTING OF HER2/NEU MARKER IN BREAST CANCER: A CORRELATIVE STUDY

Authors:

Shilpa Balar, Esha Joshi, Urja Desai

Abstract:

Currently HER-2/neu status in breast cancer is categorized by immunohistochemistry (IHC) and fluorescent in situ hybridization (FISH) in clinical practice. The amplification of the HER-2/neu gene has a prognostic significance. Quantitative determination of HER-2/neu gene amplification by qPCR or over-expression by qRT-PCR is not routinely used, whereas qPCR based assays can be an alternative method to evaluate HER-2/neu status. We searched literature and databases including the National Institutes of Health’s (NIH), National Library of Medicine (NLM), The National Center for Biotechnology Information (NCBI), PubMed, and Google Scholar. The current study aimed to identify the correlation between FISH, IHC, and real-time-PCR reaction in measuring HER-2/neu expression in breast cancer.

Keywords:

Breast cancer, FISH, HER2/neu, IHC, Quantitative PCR, Quantitative RT-PCR.

Vol & Issue:

VOL.14, ISSUE No.1, March 2022