Towards Excellence

(ISSN No. 0974-035X)
(An indexed refereed & peer-reviewed journal of higher education)
UGC-MALAVIYA MISSION TEACHER TRAINING CENTRE GUJARAT UNIVERSITY

VALIDATED HPTLC DENSITOMETRY METHOD FOR ESTIMATING QUERCETIN FROM LEAVES OF GMELINA ARBOREA ROXB

Authors:

Almutazbellah Khalid, Milan Thakar, Ridham Dave , Gaurang Sindhav

Abstract:

Gmelina arborea Roxb. (G. arborea) commonly known as White Teak. The medicinal plant is swiftly growing, belonging to the Verbenaceae family and renowned for its diverse therapeutic applications. In this study, a precise and expedient High-Performance Thin Layer Chromatographic (HPTLC) method was meticulously employed to accurately quantify quercetin from G. arborea leaves. The use of chloroform: ethyl acetate: formic acid (5:4:1) as the mobile phase on silica gel 60 F254 HPTLC plates effectively separated the components. The subsequent densitometric assessment at 254 nm unveiled a notable quercetin content (0.32% w/w) in the G. arborea methanolic (GAM) extract. The rigorous method validation through International Conference on Harmonization [ICH Q2(R1)] guidelines, encompassing linearity, the determination of the limit of detection (LOD) and quantification (LOQ), as well as precision and specificity assessments, underscored the reliability and efficacy of the method. In a nutshell, the comprehensive validation of the developed HPTLC method for the quantification of quercetin has confirmed its robustness and reliability. The meticulous assessment of linearity, specificity, precision, accuracy, and robustness aligns with ICH Q2 (R1) guidelines, attesting that the developed method is scientifically sound. This method offers a valuable analytical approach for the exploration of bioactive compounds in medicinal plants thereby contributing significantly to the advancement of quality control in botanical research.

Keywords:

Gmelina arborea Roxb., HPTLC densitometry validation, ICH Q2(R1) guidelines

Vol & Issue:

VOL.16, ISSUE No.2, June 2024