Almutazbellah Khalid, Milan Thakar, Ridham Dave , Gaurang Sindhav
Gmelina
arborea Roxb. (G. arborea) commonly known as White Teak. The
medicinal plant is swiftly growing, belonging to the Verbenaceae family and
renowned for its diverse therapeutic applications. In this study, a precise and
expedient High-Performance Thin Layer Chromatographic (HPTLC) method was
meticulously employed to accurately quantify quercetin from G. arborea
leaves. The use of chloroform: ethyl acetate: formic acid (5:4:1) as the mobile
phase on silica gel 60 F254 HPTLC plates effectively separated the
components. The subsequent
densitometric assessment at 254 nm unveiled a notable quercetin content (0.32% w/w)
in the G. arborea methanolic (GAM) extract. The rigorous method validation
through International Conference on
Harmonization [ICH Q2(R1)]
guidelines, encompassing linearity, the determination of the limit of detection
(LOD) and quantification (LOQ), as well as precision and specificity
assessments, underscored the reliability and efficacy of the method. In a
nutshell, the comprehensive validation of the developed HPTLC method for the
quantification of quercetin has confirmed its robustness and reliability. The
meticulous assessment of linearity, specificity, precision, accuracy, and
robustness aligns with ICH Q2 (R1) guidelines, attesting that the developed
method is scientifically sound. This method offers a valuable analytical approach for the exploration of
bioactive compounds in medicinal plants thereby contributing significantly to
the advancement of quality control in botanical research.
Gmelina arborea
Roxb., HPTLC densitometry
validation, ICH Q2(R1) guidelines
VOL.16, ISSUE No.2, June 2024