Krupali A Trivedi, Anaal S. Engineer, Linz-Buoy George, Hyacinth N. Highland
Objective: To assess
the synergistic potential
using an exointeractive association between Murraya koenigii
hydromethanolic leaf extract and the synthetic compound quercetin to offset
methotrexate induced toxicity in mouse cauda epididymal spermatozoa.
Methods: Healthy adult male albino
mice were divided into eight groups (N= 6/group) and were treated with
methotrexate intraperitoneally (10mg/kg body weight) at intervals of every 48 hours for
15 days, to combat this toxic effects of the drug, Murraya
koenigii hydromethanolic leaf extract (250mg/kg body weight) and quercetin
(15mg and 30mg) alone as well as in combination, were given by oral
administration. Various sperm parameters such as sperm count, motility and viability followed by Hypo-osmotic
Swelling (HOS) test, apoptosis test, Sperm Toroid Integrity (STI) and Sperm
Chromatin Dispersion (SCD) assay.
Statistical
analysis used: Statistical analysis was carried out using Graphpad
Prism Version 7 to verify levels of significance at the P < 0.001
value in one-way ANOVA.
Results: Methotrexate
induced a substantial reduction in sperm count, motility and viability which
induces apoptotic changes, also resulted in disruption of sperm toroid
protamine-DNA binding, increased fragmentation of nuclear DNA by decreased
sperm chromatin dispersion and low percentage of swollen sperm representing
membrane integrity loss. whereas Murraya koenigii hydromethanolic leaf
extract and quercetin proved effective in reversing the drug induced toxicity, especially
with the 15 mg dose of quercetin along with the plant extract brought about
significant amelioration than 30mg dose of quercetin due to its pro-oxidant
effects.
Conclusions: To reduce
the toxic manifestations of methotrexate, this exointeractive approach proved
efficient.
Exointeractive
mitigation, Intraperitoneal, Murraya koenigii, Quercetin, Hydromethanolic
VOL.15, ISSUE No.1, March 2023